phar.kindai.ac.jp
Clinical Laboratory Sciences (3rd year)
Practice of Clinical Pharmacotherapy (4th year)
Staff |
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Professor@ Shigeo Ikegawa
@ikegawaphar.kindai.ac.jp |
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Hospital Pharmaceutics (3rd year) Clinical Laboratory Sciences (3rd year) Practice of Clinical Pharmacotherapy (4th year) |
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Lecturer@ Kuniko Mitamura
@mitamuraphar.kindai.ac.jp |
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The Japanese Pharmacopoeia (2nd year) Resume of the Japanese Pharmacopoeia 1, 2 (4th year) Practice of Clinical Pharmacotherapy (4th year) |
1. Clinical proteomics of bile acid modified proteins and studies on metabolism and disposition of novel bile acid conjugates
2. Analytical studies on conjugated corticosteroids by LC/ESI-MS.
3. Development of the analytical method of oxysterol sulfates by LC/ESI-MS.
4. Mechanistic studies on the expression of toxicity and detoxification of biologically active substances of low molecules via their metabolic activation.
Today, much attention has been focused on the analyses of precise structure of the total proteins from a body fluid, particular cell line, tissue, or organism to understand specific interactions between these proteins and biologically active substances of low molecules such as drugs, steroid hormones and bile acids. We are setting forward with the dynamic studies of biologically active substances and structure elucidation of chemically modified proteins in relation to pathophysiology by the basic proteomic technique, combined with use of mass spectrometry and computer assisted programs. The aims of our researches are also feedback information of the etiological and pathophysiological knowledge for drug development and cure for disease.
y2006-z
1. Mitamura K., Sogabe M., Sakanashi H. Watanabe S., Sakai T. Yamaguchi Y., Wakamiya T., Ikegawa S., Analysis of bile acid glutathione thioesters by liquid chromatography/electrospray ionization-tandem mass spectrometry. J. Chromatogr., in press
2. Fuda H., Javitt N. B., Mitamura K., Ikegawa S., Strott C. A., Oxysterols are substrate for cholesterol sulfotransferase (SULT2B1b). J. Lipid Res., in press.
3. Sogabe M., Mitamura K., Watanabe S., Ikegawa S., Yamaguchi Y., Wakamiya T., Study on the syntheses and LC/ESI-MSn analyses of glutathione conjugates of bile acids. Peptide Sci. 2006, 43ed, 295-296 (2006).
y2005z
1. Mitamura K., Setaka M., Shimada K., Honma S., Namiki M., Koh E., Mizokami A., Determination of sulfates of androsterone and epiandrosterone in human serum using isotope diluted liquid chromatography-electrospray ionization-mass spectrometry. Biomed. Chromatogr., 19, 796-801 (2005).
2. Mitamura K., Horikawa A., Nagahama A., Shimada K., Fujii Y., Determination of digitoxin in human serum using stable isotope diluted liquid chromatography/electrospray ionization-tandem mass spectrometry. J. Liquid Chromatogr. Relat. Technol., 28, 2839-2848 (2005).
3. Mitamura K., Ogasawara C., Shiozawa A., Terayama E., Shimada K., Determination method for steroid 5?-reductase activity using liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry. Anal. Sci., 21, 1241-1244 (2005).
4. Mizuguchi T., Shibayama Y., Mitamura K., Shimada K., Contribution of glucuronic acid and sulfonic acid moieties during photocatalytic degradation of estrogen conjugates. J. Health Sci., 51, 447-452 (2005).
y2004z
1. Ishiwata S., Ozawa Y., Katayama J., Kaneko S., Shindo H., Tomioka Y., Ishiwata T., Asano G., Ikegawa S., Mizugaki M., Elevated expression level of 60-kDa subunit of tRNA-guanine transferase in colon cancer, Cancer Lett., 212, 113-119 (2004).
2. Ito H., Ishiwata S., Kosaka T., Nakashima R., Takeshita H., Negoro S., Maeda M., Ikegawa S., Enantioselective immunorecognition of protein modification with optically active ibuprofen polyclonal antibody, J. Chromatogr. B, 806, 11-17 (2004).
3. Ishiwata T., Fujii T., Ishiwata S., Ikegawa S., Naito Z., Effect of morpholino antisense oligonucleotide against lumican mRNA in human embryonic kidney (HEK) 293 cells, Path. Int., 54, 77-81 (2004).
4. Mitamura K., Narukawa H., Mizuguchi T., Shimada K., Degradation of estrogen conjugates using titanium dioxide as a photocatalyst, Anal. Sci., 20 (1), 3-4 (2004).