phar.kindai.ac.jp
Introduction to Information Science (1st)
Introduction to Development of Pharmaceuticals（2nd）
Drug Information 1 (3rd)
Information Science Practice 1 (1st)
Information Science Practice 2 (1st)
Staff |
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Professor Kazuaki Kakehi
k_kakehiphar.kindai.ac.jp |
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Introductory Pharmaceutical Sciences (1st) ) Introduction to Information Science (1st) Introduction to Development of Pharmaceuticals（2nd） Drug Information 1 (3rd) Information Science Practice 1 (1st) Information Science Practice 2 (1st) |
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Assistant Professor Mitsuhiro Kinoshita
m-kinophar.kindai.ac.jp |
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Information Science（1st） Information Science Practice 1 (1st) Information Science Practice (1st) |
1. Development of an apparatus for rapid release of oligosaccharides from glycoproteins and analysis of mucin-type glycans
2. Comprehensive analysis of oligosaccharides of cellular glycoproteins
3. Identification of disease related glycans in blood and tissue that represent potentially useful glycobiomarkers for the early diagnosis
4. Combinatorial biosynthesis of useful oligosaccharides for glycomics research
The post-genomic research era has now been launched and science has become more concerned with the proteomic approach, including the post-translational modification of proteins. However, most proteome projects have not adequately discussed the analytical and functional details of the glycosylation of proteins, lipids and proteoglycans. Therefore in order to understand biology, the introduction of concept of a more integrated form of glycobiology and glycotechnology, called "glycomics" seems appropriate. This field of research will focus on the analytical details of glycosylation as well as the functional details of glycosylation.
To understand complex life systems, it is essential to study glycan structures and functions from a genome-wide viewpoint. Our objectives are to develop useful technologies for glycomics research, construct new technologies for glycomics. Specifically, it includes the development of an automatic glycan-releasing apparatus “AutoGlycoCutter”, and the further improvement of “AutoGlycoCutter” into a high-throughput type to more rapidly and sensitively analyze O-linked glycans. Based on expression patterns and structural analyses, we investigate the relationship between disorders and structural changes of the glycans. Using this information, we aim to develop biomarkers that are specific to glycans and glycoproteins.
2006
1. Matsuno YK, Yamada K, Tanabe A, Kinoshita M, Maruyama SZ, Osaka YS, MasukoT,
Kakehi K. Development of an apparatus for rapid release of oligosaccharides at
the glycosaminoglycan-protein linkage region in chondroitin
sulfate-typeproteoglycans. Anal Biochem. in press 2006
2. Kamoda S, Ishikawa R, Kakehi K. Capillary electrophoresis with laser-induced
fluorescence detection for detailed studies on N-linked oligosaccharide profile
of therapeutic recombinant monoclonal antibodies. J Chromatogr A.
1133(1-2):332-339 (2006)
3. Matsuno YK, Nakamura H, Kakehi K. Comparative studies on the analysis of
urinary trypsin inhibitor (ulinastatin) preparations. Electrophoresis.
27(12):2486-2494 (2006)
4. Kamoda S, Kakehi K. Capillary electrophoresis for the analysis of
glycoprotein pharmaceuticals. Electrophoresis. 27(12):2495-2504 (2006)
5. Kamoda S, Nakanishi Y, Kinoshita M, Ishikawa R, Kakehi K. Analysis of
glycoprotein-derived oligosaccharides in glycoproteins detected on
two-dimensional gel by capillary electrophoresis using on-line concentration
method. J Chromatogr A. 1106(1-2):67-74 (2006)
6. Naka R, Kamoda S, Ishizuka A, Kinoshita M, Kakehi K. Analysis of total
N-glycans in cell membrane fractions of cancer cells using a combination of
serotonin affinity chromatography and normal phase chromatography. J Proteome
Res. 5(1):88-97 (2006)
7. Nakajima K, Kinoshita M, Matsushita N, Urashima T, Suzuki M, Suzuki A, Kakehi
K. Capillary affinity electrophoresis using lectins for the analysis of milk
oligosaccharide structure and its application to bovine colostrum
oligosaccharides. Anal Biochem. 348(1):105-14 (2006)
2005
1. Yasueda S, Yamakawa K, Nakanishi Y, Kinoshita M, Kakehi K. Decreased mucin
concentrations in tear fluids of contact lens wearers. J Pharm Biomed Anal.
39(1-2), 187-195 (2005)
2. Matsuno YK, Kinoshita M, Kakehi K. Fast analysis of glycosaminoglycans by
microchip electrophoresis with in situ fluorescent detection using ethidium
bromide. J Pharm Biomed Anal. 37, 429-436 (2005)
3. Kinoshita M, Kakehi K. Analysis of the interaction between hyaluronan and
hyaluronan-binding proteins by capillary affinity electrophoresis: significance
of hyaluronan molecular size on binding reaction. J Chromatogr B Analyt Technol
Biomed Life Sci. 816, 289-295 (2005)
4. Gao Y, Eguchi A, Kakehi K, Lee YC. Synthesis and molecular recognition of
carbohydrate-centered multivalent glycoclusters by a plant lectin RCA120. Bioorg
Med Chem. 13, 6151-6157 (2005)
Kamoda S, Nakano M, Ishikawa R, Suzuki S, Kakehi K. Rapid and sensitive
screening of N-glycans as 9-fluorenylmethyl derivatives by high-performance
liquid chromatography: a method which can recover free oligosaccharides after
analysis. J Proteome Res. 4, 146-152 (2005)
5. Kakehi K, Kinoshita M Carbohydrate analysis-Alteration of glycan structures in disease-, Rinsyo Kagaku、49、999-1005 （2005）
6. Kakehi K, Matsuno Y, Kinoshita M High-sensitive analysis of a fluorescent-labeled oligosaccharides using capillary electrophoresis
7. Kakehi K, Kinoshita M New evolution in glycoscience （2005）
2004
1. Kamoda S, Nomura C, Kinoshita M, Nishiura S, Ishikawa R, Kakehi K, Kawasaki
N, Hayakawa T. Profiling analysis of oligosaccharides in antibody
pharmaceuticals by capillary electrophoresis. J Chromatogr A. 1050, 211-216
(2004)
2. Matsuno YK, Kinoshita M, Kakehi K. Electrophoretic analysis of di- and
oligosaccharides derived from glycosaminoglycans on microchip format. J Pharm
Biomed Anal. 36, 9-15 (2004)
3. Gao Y, Eguchi A, Kakehi K, Lee YC. Efficient preparation of glycoclusters
from silsesquioxanes. Org Lett. 6, 3457-3460 (2004)
4. Nakajima K, Kinoshita M, Oda Y, Masuko T, Kaku H, Shibuya N, Kakehi K.
Screening method of carbohydrate-binding proteins in biological sources by
capillary affinity electrophoresis and its application to determination of
Tulipa gesneriana agglutinin in tulip bulbs. Glycobiology. 14, 793-804 (2004)
5. Nakano M, Kakehi K, Tsai MH, Lee YC. Detailed structural features of glycan
chains derived from alpha1-acid glycoproteins of several different animals: the
presence of hypersialylated, O-acetylated sialic acids but not disialyl
residues. Glycobiology. 14, 431-441 (2004)
6. Hayashi T, Yasueda S, Nakanishi Y, Ohta H, Kinoshita M, Miki Y, Masuko T,
Kakehi K. Capturing of acidic macromolecules from biological samples using a
temperature-responsive polymer modified with poly-l-lysine. Analyst. 129,
421-427 (2004)
7. Kawabata A, Nishikawa H, Saitoh H, Nakaya Y, Hiramatsu K, Kubo S, Nishida M,
Kawao N, Kuroda R, Sekiguchi F, Kinoshita M, Kakehi K, Arizono N, Yamagishi H,
Kawai K. A protective role of protease-activated receptor 1 in rat gastric
mucosa. Gastroenterology. 126, 208-219 (2004)