Research
1. Functional and genetic research of the glycoprotein-type amino acid transporters, CD98
2. Preparation, isolation and gene microarray analysis of cancer stem cells
3. Pharmaceutical research on therapeutic human antibodies against tumor antigens
4. Research of apoptosis induced by cardiac glycoside, immunosuppresants or specific antibodies
5. Molecular and histological analysis of various immune organs in the animals treated with immunosuppresants
Introduction
An organizing process that tissues, organs or bodies are formed by a germ cell is wonderful. Our laboratory purposes elucidating the question why cells grow, die or develop tumor at the molecular level. For this purpose, we try to identify and clone the gene of target protein, and then analyze a state in the gene-transfected or -knockouted cells. In addition, we are preparing a monoclonal antibody to investigate the distribution of the target protein in cells or tissues. Recently, we can make monoclonal antibodies with gene-technology and, for use, application to a diagnosis or therapy of cancer is thought about. Now, we are analyzing CD98 which is a heterodimeric 125-kDa cell surface glycoprotein, which consists of an 85-kDa heavy chain (hc) and a 40-kDa light chain (lc). This study started from the findings that an antibody for CD98 inhibited the proliferation of tumor cells. Lc has several kinds of types and goes in and out of a plasma membrane many times and forms a transporter, and hc brings lc to cell surface and regulates an amino acid transport. Recently, we found that the interaction of hc with lc contributed to the development of cancer. Pharmaceutical research on humanized therapeutic antibodies to cancer is in progress. We want to have all of you realize biological pleasure by all means.
Publication (2004-2006)
2006
1. Tokunaga M, Matsuda H, Iwahashi H, Naruto S, Tsuruoka T, Yagi H, Masuko T, Kubo M. Studies on Palauan Medicinal Herbs. IV. Immunopotentiatory activities of Ongael, Leaves of Phaleria cumingii (MEISN.) F. VILL. In diabetic mice. . J. Trad. Med.; 23: 24-26, 2006.
2. Kawabata A, Kawao N, Kitano T, Matsunami M, Satoh R, Ishiki T, Masuko T, Kanke T, Saito NColonic hyperalgesia triggered by proteinase-activated receptor-2 in mice: Involvement of endogenous bradykinin. Neuroscience Lett.: 402: 167-172, 2006.
3. Takechi, M. and Masuko, T. SBO22 You can explain immunoreaction of tumor rejection, Standard pharmaceutical series 4, Biological Pharmacy III. Host defences, 112-116, 2006.
4. Yagi, H. and Masuko, T. SBO23 You can give an outline about representative immunopotentiation methods. Standard pharmaceutical series 4, Biological Pharmacy III. Host defences 117-121, 2006.
2005
1. Onoda, F., Takeda, M., Seki, M., Maeda, D., Tajima, J., Ui, A., Yagi, H., and Enomoto, T. SMC6 is required for MMS-induced interchromosomal and sister chromatid recombinations in Saccharomyces cerevisiae. DNA Repair, 3, 429-439 (2004).
2. Yagi, H., Hidaka, M., Nakamura, M., and Masuko, T. Morphological and functional alteration of enterocytes by the daily administration of FK506: Application of UV-excited alkaline phosphatase substrate, ELF 97 phosphate. Cytometry Research, 14, 41-46 (2004).
3. Hayashi, T., Yasueda, S., Nakanishi, Y., Ohta, H., Kinoshita, M., Miki, Y., Masuko, T., and Kakehi, K. Capturing of acidic macromolecules from biological samples using a temperature-responsive polymer modified with poly-l-lysine. Analyst, 129, 421-427 (2004).
4. Kawabata, A., Itoh, H., Kawao, N., Kuroda, R., Sekiguchi, F., Masuko, T., Iwata, K., and Ogawa, A. Activation of trigeminal nociceptive neurons by parotid PAR-2 activation in rats. Neuroreport, 15, 1617-1621 (2004).
5. Nakajima, K., Kinoshita, M., Oda, Y., Masuko, T., Kaku, H., Shibuya, N., and Kakehi, K. Screening method of carbohydrate-binding proteins in biological sources by capillary affinity electrophoresis and its application to determination of Tulipa gesneriana agglutinin in tulip bulbs. Glycobiology, 14, 793-804 (2004).
6. Yagi, H., and Masuko, T. Expression of CD98 amino acid transporter on murine haematopoietic stem cells. Cytometry Research, 14, 57-62 (2004).
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