Biochemistry

Staff

Professor　 Seiji Ichida 　 seijiphar.kindai.ac.jp
	Biochemistry (1st ) Laboratory for Fundamental Pharmaceutical Sciences (1st ) Research Course for New Pharmaceutical Sciences (1st ) Laboratory for Biological Chemistry and Physiology(2nd ) Life science (Other Faculty)

Associate Professor　 Tetuyuki Wada, 　tetsupharphar.kindai.ac.jp
	Fundamental Biochemistry (1st ) Seminar for Biology (1st ) Laboratory for Fundamental Pharmaceutical Sciences (1st ) Research Course for New Pharmaceutical Sciences (1st ) Laboratory for Biological Chemistry and Physiology (2nd )

Assistant　Yoshinori　 Funakami　funakamiphar.kindai.ac.jp
	Laboratory for Pharmacology (3rd ) Laboratory for Fundamental Pharmaceutical Sciences (1st ) Research Course for New Pharmaceutical Sciences (1st )

Research

1. An investigation into the expression and role of voltage-sensitive ionic channels on the developmental processes to neuronal cells.
2. An investigation into the characteristics of calcium channels in the neuronal cells by forced expression of neural L-or N-type calcium channels
3. An investigation into the characteristics of natural bioactive substances which affect the signal function in neural cells

Introduction

We have been investigating the functional role of calcium ion channels in neuronal cells since our laboratory was started. Therefore, we think that if the mechanisms by which the bioactive resources affect the signal function (which particularly includes the mobilization of calcium ions and the function of calcium ion channels) were able to make clear, it would have a chance to create a new type of medicine against a disease which is due to cause the irregular for the signal function in the neuronal cells

Details for our investigation are as follows.
1. An investigation into the expression and role of voltage-sensitive ionic channels on the developmental processes to neuronal cells. The neuroblastoma ? glioma hybrid NG108-15 (NG) cell line is used as a suitable model system to investigate the mechanisms of neuronal development and differentiation. Therefore, the functional change for up-regulated voltage-sensitive sodium and calcium channels is investigated after differentiated NG cells with dibutyryl cAMP (Bt2cAMP) by the methods of whole-cell patch clamp and immunocytochemistry. (Collaboration with Prof. Shigeru Yoshida, Department of Life Science, School of Science & Engineering, Kinki University)
2. An investigation into the characteristics of calcium channels in the neuronal cells by forced expression of neural L-or N-type calcium channels. The cells forced expression with neural L-or N-type calcium channels are used to investigate the difference in a signal regulation between the two channels by methods of whole-cell patch clamp and Ca2+ imaging with a Ca2+-sensitive dye for monitoring [Ca2+]i. [Collaboration with Prof. Shigeru Yoshida and Prof. Yasuo Mori (Faculty of Engineering, Kyoto University)]
3. An investigation into the characteristics of bioactive substances from natural resources which are able to affect the signal function (which particularly includes the mobilization of calcium ions and the function of calcium ion channels) in neuronal cells. It is important to investigate the pharmacological action mechanism for the bioactive resources, because if the mechanisms by which the bioactive resources affect the signal function were able to make clear, it would have a chance to create a new type of medicine against a disease which is due to cause the irregular for the signal function in the neuronal cells.

Publication (2004-2006)

2006
1.　Takashi Imanishi, Kayoko Matsushima, Akinori Kawaguchi, Tetsuyuki Wada, Takashi Masuko, Shigeru Yoshida and Seiji Ichida, Enhancement of veratridine-induced sodium dynamics in NG108-15 cells during differentiation, Biol Pharm Bull. 29, 701-4, 2006
2.　Takashi Imanishi, Kayoko Matsushima, Akinori Kawaguchi, Tetsuyuki Wada, Shigeru Yoshida and Seiji Ichida, Enhancement of serotoin- and bradykinin-evoked calcium ion dynamics in differentiated NG108-15 cells, Neurosci. Lett., in press Neurosci Lett., 405(1-2), 1-4, 2006
3.　Takashi Imanishi, Kayoko Matsushima, Akinori Kawaguchi, Tetsuyuki Wada, Shigeru Yoshida and Seiji Ichida, Increased response to high KCl-induced elevation in the intracellular-Ca2+ concentration in the differentiated NG108-15 cells and the inhibitory effect of the L-type Ca2+ channel blocker. Neurochem. Res., 31, 33-40, 2006
2005
1.. Takashi Imanishi, Kayoko Matsushima, Akinori Kawaguchi, Tetsuyuki Wada, Takashi Masuko, Shigeru Yoshida and Seiji Ichida, Enhancement of veratridine-induced sodium dynamics in NG108-15 cells during differentiation, Biol. Pharm. Bull., in press (accepted on 5 January, 2006)
2. Takashi Imanishi, Kayoko Matsushima, Akinori Kawaguchi, Tetsuyuki Wada, Shigeru Yoshida and Seiji Ichida, Enhancement of serotoin- and bradykinin-evoked calcium ion dynamics in differentiated NG108-15 cells, Neurosci. Lett., in press (accepted on 12 December 2005)
3. Takashi Imanishi, Kayoko Matsushima, Akinori Kawaguchi, Tetsuyuki Wada, Shigeru Yoshida and Seiji Ichida, Increased response to high KCl-induced elevation in the intracellular-Ca2+ concentration in the differentiated NG108-15 cells and the inhibitory effect of the L-type Ca2+ channel blocker. Neurochem. Res., in press (accepted on 6 October 2005)
4. Tetsuyuki Wada, Takashi Imanishi, Akinori Kawaguchi, Masayuki X. Mori, Yasuo Mori, Keiji Imoto and Seiji Ichida, Effects of calmodulin and Ca2+ channel blockers on ω-conotoxin GVI A binding to crude membranes from α1B subunit (Cav2.2) expressed BHK cells and mice brain lacking the α1B subunits, Neurochem. Res. (accepted on 28 June 2005)
5. Seiji Ichida, Junichi Abe, Kuniyo Komoike, Takashi Imanishi, Tetsuyuki Wada, Takashi Masuko* and Takeshi Minami, Characteristics of omega-conotoxin GVI A and MVIIC binding to Cav 2.1 and Cav 2.2 channels captured by anti-Ca2+ channel peptide antibodies, Neurochem. Res. (accepted on 18 March 2005).
2004
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